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non small cell lung cancer nsclc cell lines a549  (ATCC)


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    ATCC non small cell lung cancer nsclc cell lines a549
    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
    Non Small Cell Lung Cancer Nsclc Cell Lines A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 35640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non small cell lung cancer nsclc cell lines a549/product/ATCC
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    non small cell lung cancer nsclc cell lines a549 - by Bioz Stars, 2026-03
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    1) Product Images from "Epithelial plasticity shapes intratumoral heterogeneity and cell lineages in early-stage lung cancer"

    Article Title: Epithelial plasticity shapes intratumoral heterogeneity and cell lineages in early-stage lung cancer

    Journal: Science Advances

    doi: 10.1126/sciadv.ady8546

    ( A ) Box plots showing KLF4 mRNA expression in the A549 LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
    Figure Legend Snippet: ( A ) Box plots showing KLF4 mRNA expression in the A549 LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.

    Techniques Used: Expressing, Over Expression, Control, Viability Assay, Migration, Soft Agar Assay, Marker



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    non small cell lung cancer nsclc cell lines a549  (ATCC)
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    ATCC non small cell lung cancer nsclc cell lines a549
    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
    Non Small Cell Lung Cancer Nsclc Cell Lines A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a549 non small lung cancer cells  (ATCC)
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    ATCC a549 non small lung cancer cells
    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
    A549 Non Small Lung Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell culture non small cell lung cancer nsclc cell line nci h1944  (ATCC)
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    ATCC cell culture non small cell lung cancer nsclc cell line nci h1944
    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
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    non small cell lung cancer cell line a549  (ATCC)
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    ATCC non small cell lung cancer cell line a549
    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
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    non small cell lung cancer cell line a549 - by Bioz Stars, 2026-03
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    ATCC human non small cell lung cancer cell line
    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
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    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
    Non Small Cell Lung Cancer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non small cell lung cancer/product/ATCC
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    human non small cell lung cancer cell line a549  (ATCC)
    99
    ATCC human non small cell lung cancer cell line a549
    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
    Human Non Small Cell Lung Cancer Cell Line A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human non small cell lung cancer cell line a549/product/ATCC
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    remarkable anti proliferative activity against non small cell lung cancer cell lines  (ATCC)
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    ( A ) Box plots showing KLF4 mRNA expression in the <t>A549</t> LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.
    Remarkable Anti Proliferative Activity Against Non Small Cell Lung Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    small cell lung cancer cell lines nci h146  (ATCC)
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    ATCC small cell lung cancer cell lines nci h146
    (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in <t>NCI-H146.</t> N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .
    Small Cell Lung Cancer Cell Lines Nci H146, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small cell lung cancer cell lines nci h146/product/ATCC
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    human non small cell lung cancer cell lines h460  (ATCC)
    99
    ATCC human non small cell lung cancer cell lines h460
    (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in <t>NCI-H146.</t> N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .
    Human Non Small Cell Lung Cancer Cell Lines H460, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human non small cell lung cancer cell lines h460/product/ATCC
    Average 99 stars, based on 1 article reviews
    human non small cell lung cancer cell lines h460 - by Bioz Stars, 2026-03
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    Image Search Results


    ( A ) Box plots showing KLF4 mRNA expression in the A549 LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.

    Journal: Science Advances

    Article Title: Epithelial plasticity shapes intratumoral heterogeneity and cell lineages in early-stage lung cancer

    doi: 10.1126/sciadv.ady8546

    Figure Lengend Snippet: ( A ) Box plots showing KLF4 mRNA expression in the A549 LUAD cell line in the overexpression group and the control group. The median and interquartile range are shown for each cluster, two-sided Wilcoxon test. ( B ) Cell viability assay showing that overexpression of the KLF4 gene in KLF4 low LUAD A549 cells decreases cell proliferation. ( C ) Colony formation assays confirming that overexpression of the KLF4 gene decreases cancer cell proliferation in the A549 cells. ( D ) Transwell assays confirmed that overexpression of the KLF4 gene decreases cell migration in the A549 cells. ( E ) Soft agar assay showing that overexpression of the KLF4 gene inhibits anchorage-independent growth in the A549 cells. ( F and G ) Growth curves of xenograft tumors (F) and subcutaneous tumor size (G) of each group showing that the overexpression of the KLF4 gene inhibits tumor growth. ( H ) CUT&Tag-seq tracks of the gene of transitional marker and C2 cluster–specific genes locus in the indicated cells. KLF4-OE: Flag-KLF4–overexpressing A549 cells (anti-Flag antibody); Ctrl: Flag-overexpressing A549 cells (anti-Flag antibody). ( I ) Volcano plot showing the transitional marker and C2 cluster–specific genes distribution with respect to KLF4 expression levels. ( J and K ) GSEA showing that the KLF4 + cancer cell (J), AT1 cell, and AT2 cell (K) features were affected by high expression of KLF4.

    Article Snippet: Human embryonic kidney (HEK) 293T cells [American Type Culture Collection (ATCC), catalog #ACS-4500) and the non–small cell lung cancer (NSCLC) cell lines A549 (ATCC, catalog #CCL-185) and NCI-H1437 (ATCC, catalog #CRL-5872) were used.

    Techniques: Expressing, Over Expression, Control, Viability Assay, Migration, Soft Agar Assay, Marker

    (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in NCI-H146. N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .

    Journal: bioRxiv

    Article Title: Nanobody MET CAR T cells show efficacy in solid tumors

    doi: 10.64898/2026.01.27.702111

    Figure Lengend Snippet: (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in NCI-H146. N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .

    Article Snippet: Small cell lung cancer cell lines NCI-H146 (ATCC HB173) and NCI-H1341(ATCC CRL-5864) were cultured in DMEM-F12 supplemented with 10% FBS, 1X Insulin-Transferrin-Selenium and 1% penicillin/streptomycin.

    Techniques: Western Blot, Expressing, Flow Cytometry, Cytotoxicity Assay